![]() ![]() These properties make the ciliated protozoan an appropriate organism to determine the health of an aquatic environment. Additionally, they are able to consume free organic material from the environment if necessary. They play an integral part in the community by connecting the food chain between bacteria and small phytoplankton to larger metazoa and zooplankton. The optimized methods reported in this paper provide scientists with a convenient tool to perform cell counting for Tetrahymena ecotoxicity assessment.Ĭiliated protozoa are unicellular eukaryotes commonly found in aquatic environments. Among the five methods tested, all of them could yield decent results, but the deep-learning-based SDM displayed the best performance for Tetrahymena cell counting. We conducted Tetrahymena number measurement using five different methods: particle analyzer method (PAM), find maxima method (FMM), trainable WEKA segmentation method (TWS), watershed segmentation method (WSM) and StarDist method (SDM), and compared their results with the data obtained from the manual counting. In this study, we established the ImageJ-based workflow to quantify ciliate numbers in a high-throughput manner. Although advanced counting devices are available, the specialized and usually expensive machinery precludes their prevalent utilization in the regular laboratory routine. You might also want to try the ‘Convoluted Background Subtraction’ tool available in the BioVoxxel Toolbox, which appears to do a better job at suppressing/removing the halos surrounding your stained cells, which potentially helps with the subsequent thresholding and particle selection.Previous methods to measure protozoan numbers mostly rely on manual counting, which suffers from high variation and poor efficiency. Or you could smooth the particles on the thresholded image using binary filters to remove irregularities in the particles shape that cause the watershed algorithm to split single particles (I would expect single nuclei to be pretty smooth, round objects). So, the watershed step might not be required for your analysis.Īlternatively, you could try to use the ‘Irregular Watershed’ tool available in the Biovoxxel Toolbox ( BioVoxxel Toolbox). Looking at your sample image, the nuclei appear to be quite sparse with hardly any nuclei directly touching each other. When you say that you ‘can see lines within the cells’ that is due to the watershed and occurs if the structure has an irregular shape that is split by the watershed operation, which tries to separate touching particles. Screenshot of detected particles shown on original image: Run("Analyze Particles.", "size=5-150 circularity=0.65-1.00 display clear add") Volko run("Subtract Background.", "rolling=25") Either way, I don’t think that you will get any meaningful cell count from that clump and it is probably best excluded from the analysis. I assume that this is either some dirt or a larger clump of cells. The difficulty is the clump of material in the bottom right corner of the image. The code below hopefully gets you a bit closer to what you are aiming for. When you do the watershed, the options in the ‘Binary Options’ are also set such that the watershed is carried out on the black background rather than the white particles that you thresholded.įinally, your image doesn’t appear to be calibrated (scale set to 1 pixel = 1 inch). At the moment, you are selecting the halo around the centres of the stained structures rather than their centre - not sure whether this is your intention. How did you stain and image these nuclei?Īnyway, to threshold the bright spots that form the centre of your particles (I assume that this is what you try to count), I would suggest not to select the ‘Light Background’ option when you do the background subtraction. The stained particles also appear to have some rather strange halos more like a phase contrast image than a fluorescent image. Looking at your image, it looks a bit unusual for fluorescently labeled nuclei - I would expect the contrast of the nuclei relativeto the background to be considerably higher for most of the conventional fluorescent nuclei stains (e.g. ![]()
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![]() Points can be used to redeem Team Rubicon hoodies, t-shirts, boonies, tumblers and MTN DEW x Team Rubicon decals. Like Team Rubicon, we know Dew Nation has a bias for action and we’re excited to see how they’ll step up to match DEW’s $1 million commitment to our mission.”ĭuring the promotion running now through March 24, consumers can make a monetary donation to Team Rubicon at the DEW Nation Rewards Team Rubicon store and in return earn exclusive MTN DEW x Team Rubicon rewards and gear, including t-shirts, backpacks, sleeping tents – and for the biggest Team Rubicon supporters, a once-in-a-lifetime race-day experience with Dale Earnhardt Jr.Ĭonsumers can also represent the MTN DEW x Team Rubicon mission by purchasing participating MTN DEW products and redeeming for points at During the promotion period, consumers will earn two times the DEW Nation Reward points by purchasing participating products. “DEW’s contribution will enable us to train more volunteers in critical skills, respond to more disasters around the world, and help more people on their worst day. “We’re honored and humbled by this major commitment from MTN DEW in support of our mission,” said Jake Wood, Co-Founder and CEO of Team Rubicon. will travel with MTN DEW to a Team Rubicon worksite in the Florida Panhandle to work alongside the volunteers as they rebuild a community that was ravaged by Hurricane Michael in 2018. Team Rubicon was there to support and rebuild those affected areas, and I’m proud to team with Mountain Dew to support its mission,” said Earnhardt Jr. ![]() “Natural disasters have hit close to home for me – like Hurricane Florence’s impact on the Carolinas. They are the true embodiment of what it means to ‘DO THE DEW,’ and we want to fuel that fire in all of DEW Nation to support this worthy mission.” DEW Nation HQ Launch Challenge starts today Click the link below for a personal referral code to share with your friends & family for the. “The volunteers of this great organization, many of them military veterans, continue to serve our country through disaster response teams. Finally more ways to get points on a daily basis ( i miss dew smash giving points) I found an easy way to get points on the puzzle so basically all you have to do is match 1. “Team Rubicon is an extraordinary organization, and we are honored to partner with them and leverage our platform to raise awareness of its mission to aid in disaster-stricken areas,” said Nicole Portwood, vice president, marketing, MTN DEW. More ways to earn points on Dew nation Hq So we got a new game dew holiday jigsaw like with the other games you can play it five times a day and get 5 points per round. To kick off the partnership, the brand announced a $1 million donation to fuel Team Rubicon’s efforts, and has teamed with longtime DEW® spokesperson and Team Rubicon supporter Dale Earnhardt Jr. to help rally DEW Nation to participate in the MTN DEW x Team Rubicon mission. We refer to those products, services and websites collectively as the Services in this policy. In an epic rallying cry to get out and do, MTN DEW® is teaming up with Team Rubicon, an organization that uses the skills and experiences of military veterans and first responders to serve communities impacted by disasters. DEW® Nation HQ Privacy Policy Effective Date: FebruThis Privacy Policy applies to all the products, services and websites offered by Influitive Corporation and its subsidiaries and affiliates, except where otherwise noted. ![]() ![]() ![]() They were ultimately successful in repelling the invaders, although at the cost of permanently cutting Earth off from interstellar travel. Faced with mutual extermination, the Vasudans and humans quickly ended their hostilities and formed a pact to fight against the Shivans. All attempts to communicate, let alone negotiate, were met with silence. The galactic balance of power was soon shifted, however, with the appearance of the Shivans, a third race with highly advanced technology that quickly started cutting a swath through the opposing forces. The first game in the series, the awkwardly titled Descent: Freespace - The Great War, an offshoot of the popular Descent series of free-flight 3D corridor crawlers, told the story of the eponymous Great War between Terrans (humans) and an aggressive race of aliens called the Vasudans. Hunting down enemy ships in the middle of a nebula was one of the most unique aspects of Freespace 2. Although there are still blips on the proverbial radar, such as X2: The Threat and Freelancer, it seems that the sun has set on one of the premier gaming genres of the 1990s, a decade that brought us a number of fantastic entries into the Wing Commander and X-wing series, and which was capped off by one of the finest PC games ever (of any genre), Freespace 2. Sci-fi flight sim games are deader than a doornail, at least on the PC. Luckily, even though it's six years old, Freespace 2 still looks and plays as well as it ever did-if you can find a copy, that is. With the general distaste for niche products and games that require peripherals to run (I'd wager that the percentage of PC gamers that also own joysticks is as low as it has ever been), it seems unlikely that we'll ever see a space sim of the scope and quality of Freespace 2 ever again. (It's worth noting, however, that there is a Babylon 5 total modification for the Freespace 2 engine that updates the graphics and includes a lengthy single-player campaign.) ![]() The project never did see the light of day, despite the usual round of online petitions and uproar from the Internet Babylon 5 community. The developers continued to work on it for a year, hoping to buy the license from Sierra, but to no avail. Despite the fact that the company had spent a ton of money on the game in the two years of development, and that it was only months away from release, the Sierra bigwigs decided that they weren't going to recoup their investment in the game and decided to cancel the project. The nail in the coffin of the big-budget space sim was probably Sierra's cancellation of its high-profile Babylon 5 game late in 1999. It doesn't look like the space sim genre is going to come back in any big way in the future, though. Thanks to mods, Freespace 2 and Babylon 5 live on well past their commercial extinction. While the controls actually worked out well, nothing can really compare with the feel of a good force feedback joystick kicking in your hands while you gun down enemy ships. Freelancer has a rather large asterisk by its name, anyway, due to the fact that it was based around a mouse-and-keyboard interface and didn't even include support for joysticks. Space sims, however, have almost disappeared completely, with the titles that are being released, such as X2: The Threat and Freelancer, doing rather poorly at retail. ![]() The general- audience flight sim genre as a whole has become a relatively niche category that most major publishers avoid, save for Microsoft and its Flight Simulator and Combat Flight Simulator series. It's rather unfortunate that sci-fi flight sims have become so rare over the past half decade. ![]() ![]() In December 2012, it was announced that Hakim would join the BBC. īeginning in 2011, Hakim co-hosted Dateline with fellow video journalist Mark Davis, the two taking it in turns to present the programme in approximately six-week blocks, while the other was away on assignment. She was awarded the United Nations Association of Australia Media Peace Prize for best Australian Television News Coverage in 2009, and has also been a finalist for the Walkley Young Australian Journalist of the Year Award. Hakim returned to Kabul for her first Dateline report in 2008, and from then reported from across the world for Dateline and SBS World News Australia. īesides English, Hakim is fluent in Persian, Dari, Hindi, Urdu, and Pashto, and as of 2022 was learning Mandarin. She then went on to study a Bachelor of Arts in Journalism via distance education at Monash University from 2007 to 2009, in addition to a cadetship at the Special Broadcasting Service. ![]() In 2005, Hakim received a Diploma in Journalism at Macleay College, Sydney. Hakim went on to study for a Bachelor of Arts degree in Media from 2002 to 2004 at Macquarie University, where she was also a board member of the Macquarie University Union. She also attended Parramatta West Public School. Hakim attended Macarthur Girls High School in Parramatta, Sydney, where she played the violin and became a sports captain and prefect. After two years in Pakistan, the family settled in Australia as migrants in 1986, when she was three years old. The family travelled on horseback overland to Pakistan with the help of people smugglers. ![]() Her family fled the country during the Soviet–Afghan War when she was six months old. Hakim was born on 26 June 1983 in Kabul, Afghanistan. ![]() She started her career at SBS Television, moving to BBC TV in 2012. After her family left Afghanistan and settled in Australia in 1986, she grew up in the western Sydney suburb of Parramatta and went on to study journalism. She is currently one of the chief presenters at BBC News broadcasting in English in the UK and globally. Yalda Hakim (born 26 June 1983) is an Australian broadcast journalist, news presenter, and documentary maker. ![]() ![]() ![]() Meanwhile, I just got all these things in my regular playing, then I crafted them for XP, and I doubled the amount. ![]() That means I can get up to 160 glowing blood backs. Where I'm at, I can do RR and walk out with anywhere from 40-80 glowing blood. I always use glowing blood packs as a good reference: I don't use stimpacks, I used to always do Tea Time, so I had a lot of tick blood. Some of us are more akin to Walmart whole others are that little mom/pop shop. Your gonna have stronger experienced players who sell things for cheap because it's cost effective for them, whole others may see the effort they're putting into finding/crafting something as worth more caps. When it comes to player vendors, it much like the real world. Prime example: the 3 rare colored baseball bats, they have a near nothing drop rate and are incredibly rare, but they coat the same amount as any other bat at a vendor. So when it comes to items the in-game value isn't exactly reliable, it's more what the value to a protectron vendor is imo. r/galaxynewsradio - Fallout-sounding music r/falloutcosplayers - Fallout-related cosplay r/Wasteland - A subreddit for the Wasteland games. r/thefalloutdiaries - Journal-like fan fiction. r/falloutmods - Fallout modding community r/classicfallout - Classic Fallout Games r/falloutlore - The lore of the Fallout series Removal or approval of all posts and comments in the end come down to moderator discretion. These include trading posts, "quitting" posts, and subjects on the Bethesda Plz wiki-page (a collection of oft-repeated, well-received suggestion posts for Bethesda). Do not name and shame in general.ĭo not make disallowed posts. Do not accuse players of cheating, use Bethesda's official support pages. Do not ask users to go after other players in game. exe files, pirated mods, pirated keys, third party market resellers, game crashing cheats, duplication exploits, etc. This includes but is not limited to: torrents, free full-game downloads. No promotion or enabling of pirated content and game exploits. Only upon moderator verification can you post it. If you have evidence, you are welcome to submit it, in confidence, to the unaffiliated moderators for verification. Second-hand information or word of mouth does not constitute as evidence. Avoid the use of trailing ellipsis.ĭo not post rumors or leaks without actual evidence. ![]() If you're posting a question, put it in the title and give further information inside. ![]() If the average user has to click on your post to understand the context, then it is not descriptive enough. Use descriptive titles when submitting a post. More than 1/10 of your posts or comments being self-promotional is spamming. Always follow Reddit guidelines for self-promotion when sharing your own content. Absolutely no harassment, witchhunting, sexism, racism or hate speech will be tolerated.ĭo not spam. Keep it civil, do not make personal attacks to other users, even if they initiated it. Comments are fine.įollow proper Reddiquette when submitting and commenting. This is not limited to image macros, it also applies to popular trends of any kind. Use spoiler tags when commenting sensitive information. Before you ask, please read this guide to returning to the game and/or if it's worth buying.ĭo not post spoilers in titles, period. ![]() ![]() Check and process your inboxes once a day.Instead, become the master of your inboxes. This will create a huge backlog of stuff for you to go through, and it will definitely stress you out. This stage will sound familiar to my long-time readers, but it should be covered here: Don’t allow your inboxes to overflow. Read a lot of blogs? Put them all into a feedreader, in a single stream of posts, instead of having to check 25 different inboxes. At work, have one inbox for all incoming paperwork. Get different voicemails? Try forwarding them to one service, or use an internet service to deliver them to your email inbox. Have four email services? See if you can forward them all to one service. ![]() For example, how many places in your home do incoming papers get placed? Have one inbox at home for all mail, papers from work, school papers, phone notes, computer printouts, schedules, and more. For all the rest, see if you can combine multiple information streams into one inbox. See if you can go a week without missing it. If something’s not giving you value, consider eliminating it from your life.
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Strange Sorcerer Supreme 9-13 and 15 Tell us what you think about Dr. Strange Sorcerer Supreme 14-18 Book of Vishanti Backup story in Dr. Strange comic books: Doctor Strange #62 Dr. Leslie gives his reaction to Sam Raimi's Doctor Strange in the Multiverse of Madness and the gang takes a look at some classic Dr. On today's Struggle Session Jack and Leslie are joined by special guests Aaron Thorpe and Elana Levin for a round table discussion on the Sorcerer Supreme Dr. Hear episodes like this when they release and support the show by subscribing! or or One Dr. Visit /adchoices This show is part of the Spreaker Prime Network, if you are interested in advertising on this podcast, contact us at Guys: Vince Return and Merger and WrestleMania: CNBC appearance: Shane McMahon injury: Cody Rhodes Big Entrance: Bret Hart Comments on modern wrestling: Dante Martin Injury (Gruesome): Learn more about your ad choices. We also discuss how the current state of the blockbuster action movies in the wake of John Wick and Top Gun, and debate safety in pro wrestling. struggle to use their data assets effectively due to poor data quality. Support the show and get hundreds of bonus episodes and Bryan Quinby of the hit new podcast Guys joins us to discuss the endless out-of-the-ring drama of the WWE including the return of Vince McMahon, post-WrestleMania fan anger, and the WWE/UFC merger. A: Yes, the full recording of the AWS Online Tech Talk, Fuzzy Matching and. Listen to Struggle Session instantly on your tablet, phone or browser - no downloads needed. We talk video games, movies, TV, wrestling, comic books, music, anime, and all of your problematic faves. Leslie Lee III and Jack Allison wade into the reactionary hellhole of modern America. ![]() How did the master of comics plan to kill off the modern super hero fad in its infancy? Get bonus episodes like this when they release by subscribing: Find out here: Intro: Outro: Brought To Light by Alan Moore & Gary Lloyd Check out our new merch here: Send a voicemail here: Have a question or a take? Send in a 120 seconds or less clip of your take to This show is part of the Spreaker Prime Network, if you are interested in advertising on this podcast, contact us at Struggle Session is the world's finest politics and pop culture podcast. On today's episode Jack, Leslie, and Aaron discuss the pitch document for Alan Moore's unpublished DC Superhero epic Twilight of the Superheroes. ![]() ![]() ![]() Most of the software we know fall into the first category. Then, you would potentially have to re-enter some of your data in Ancestris. This is quite rare, but in this case, Ancestris will not be able to read the file, and you will have to fix the gedcom file manually. In the worse case scenario, your current software will not be able to export some important data or even worse will not respect enough the Gedcom format which will generate reading errors later, preventing the file to be loaded.However, genealogy software may generate 3 types of anomalies: Generally, your current software will be able to export most of your data in the Gedcom format. That is why Ancestris chose to be 100 % compliant with the Gedcom standard! You might face two different situations when migrating: This can result in data loss during exchanges between genealogists. However, many programs do not scrupulously comply with this Gedcom standard: they make specific choices with the way they organize their data, which makes it later difficult to convert it to the Gedcom format. So transferring your data could be very easy if your current software did its job perfectly. This format is an international standard for information exchange between genealogy software. On the other hand, recovering all your data at the same place could be much harder to manage depending on your current software.Īncestris is here to make it easy for you.Īncestris is able to read any file in Gedcom format. After a few hours using it, you'll get used to it and that is it! It is just a matter of getting used to Ancestris and its interface : 'What is the name of that action?', 'Where is that thing again ?', 'How do we do that?', and so on. Recover all your data during the transition process.Forego the software you are currently using and learn another one. ![]() ![]() ![]() Sharps can be added by using the "#" symbol after the note letter, and flats by using the letter "b".įor details on how to use the "javascript" and "javascript-file" commands, please refer to the JAVASCRIPT.md documentation file. By setting the octave for middle C, the note name range can be changed. Where notes can be provided as arguments, they can also be written as note names, by default from C-2 to G8 which corresponds to note numbers 0 to 127. If ReceiveMIDI can't find the exact name that was specified, it will pick the first MIDI output port that contains the provided text, irrespective of case. The MIDI device name doesn't have to be an exact match. Tune-request quiet javascript javascript-fileīy default, numbers are interpreted in the decimal system, this can be changed to hexadecimal by sending the "hex" command.Īdditionally, by suffixing a number with "M" or "H", it will be interpreted as a decimal or hexadecimal respectively. System-exclusive system-exclusive-file time-code song-position song-select Pitch-bend system-realtime continue active-sensing reset system-common Note-numbers octave-middle-c note-on note-off poly-pressure control-changeĬontrol-change-14 nrpn-full rpn-full program-change channel-pressure Read commands from standard input until it's closedĪlternatively, you can use the following long versions of the commands:ĭevice virtual pass-through decimal hexadecimal channel timestamp version Print version information and exit h or -help Print Help (this message) and exit Jsf path Execute the script in this file for each message Js code Execute this script for each received MIDI message Q Don't show the received messages on standard output Pc (number) Show Program Change, optionally for program (0-127) Rpnf (number) Show full RPN (MSB+LSB), optionally for parameter (0-16383) Rpn (number) Show RPN, optionally for parameter (0-16383) Nrpnf (number) Show full NRPN (MSB+LSB), optionally for parameter (0-16383) Nrpn (number) Show NRPN, optionally for parameter (0-16383) Pp (note) Show Poly Pressure, optionally for note (0-127)Ĭc (number) Show Control Change, optionally for controller (0-127)Ĭc14 (number) Show 14-bit CC, optionally for controller (0-63) Off (note) Show Note Off, optionally for note (0-127) On (note) Show Note On, optionally for note (0-127) ![]() Omc number Set octave for middle C, defaults to 3 Nn Output notes as numbers instead of names Ts Output a timestamp for each received MIDI message Hex Interpret the next numbers as hexadecimals by defaultĬh number Set MIDI channel for the commands (0-16), defaults to 0 Pass name Set name of MIDI output port for MIDI pass-throughįile path Loads commands from the specified program fileĭec Interpret the next numbers as decimals by default Virt (name) Use virtual MIDI port with optional name (Linux/macOS) If you're using the macOS Homebrew package manager, you can install ReceiveMIDI with:ĭev name Set the name of the MIDI input port Just take a look into the Builds directory when you download the sources. Since ReceiveMIDI is free and open-source, you can also easily build it yourself. You can download pre-built binaries from the release section: Here's a tutorial video about both SendMIDI and ReceiveMIDI, including some tips and tricks of how to use the command-line on macOS: By providing filter commands, it's possible to only focus on particular MIDI messages. This tool is mainly intended for quickly monitoring the messages that are sent to your computer from a particular MIDI device. MIDI.read() // Continuously check if Midi data has been received.ReceiveMIDI is a multi-platform command-line tool makes it very easy to quickly receive and monitor MIDI messages from MIDI devices on your computer.Īll the heavy lifting is done by the wonderful JUCE library. The source code is available as Open Source, under the BSD license. MIDI Monitor is FREE to download and use. Musicians will find it handy for tracking down confusing MIDI problems, and programmers can use it to test MIDI drivers and applications. MIDI.begin(MIDI_CHANNEL_OMNI) // Initialize the Midi Library. MIDI Monitor is an app to display MIDI signals going in and out of your Mac. PinMode (LED, OUTPUT) // Set Arduino board pin 13 to output Create an instance of the library with default name, serial port and settings #define LED 13 // Arduino Board LED is on Pin 13 I could really use some guidance on fixing this problem.īelow is also the code that I'm using: #include // Add Midi Library Unfortunately, every time I play a note on the keyboard the Arduino doesn't respond with the blink of the LED. I am also using this software called MIDIKeys as a virtual keyboard for the MIDI notes. I am following this circuit as my guideline and am sending MIDI messages via MIDI interface with the use of this cable. So I am currently creating a MIDI to CV module and am still having trouble with my MIDI input circuit. ![]() ![]() ![]() ![]() Search for Markdown guide or Markdown cheatsheet, and you’ll be rewarded with many sites that can help teach you. This isn’t a lesson on using Markdown, as there are far better instructional sites to do that. Markdown is portable across platforms and all Markdown interpreters, and even if Markdown itself goes out of favor, it’s still human-readable. Using proprietary tools like Microsoft Word or Apple Pages creates text files that aren’t portable to other tools, and can cause problems sharing across platforms. That sounds like a bold statement, but since it’s a plain-text format the statement is accurate. While Markdown might seem like a fad, and its fans are sometimes almost cultish in their adoration, Markdown itself is something to consider because it’s future-proof. I’m crazy about the messaging app Telegram, and I use Markdown in it all the time to format what I’m writing. One great place to experiment with Markdown is in our Slack ( /slack), because Slack also has Markdown support. Markdown can do some really complex things like creating tables in a super-easy way, but you don’t have to exercise all of the features of this little language to still get some benefits from it. You may want to make a comment on a blog or fill out a description in an online form and you can use Markdown to format your text as bold, underlined, or italics, and to add bulleted and numbered lists. Because Markdown is so popular amongst nerds, they’ve built Markdown support into lots of tools you use every day. You might be thinking this isn’t interesting to you because you don’t write a blog, but there are reasons to learn a smidge of Markdown even for normal people. Markdown is a plain-text format that simplifies writing for the web and is easier to read than HTML. ![]() I don’t know if you’ve gotten onto the Markdown train yet, but I’ve been enjoying it more and more over the years since Bart first started nagging me to use it. Editor and Preview Pane Showing Formatting in MacDown ![]() |